BioRoot RCS Extracts Modulate the Early Mechanisms of Periodontal Inflammation and Regeneration

摘要: Abstract Introduction The balance between periapical tissue inflammation and regeneration after the removal of necrotic/infected tissues is pivotal in determining success endodontic treatment. This study was designed to investigate effect silicate-based root canal sealer BioRoot RCS (BRCS; Septodont, Saint-Maur-des-Fosses, France) on modulating inflammatory mechanisms early steps initiated by human periodontal ligament (PDL) fibroblasts. Methods Samples BRCS Pulp Canal Sealer (PCS; SybronEndo, Orange, CA) were incubated culture medium obtain material extracts. To simulate bacterial infection use, PDL fibroblasts stimulated with lipopolysaccharides cultured secretion proinflammatory cytokine (interleukin 6) growth factor (transforming beta 1) quantified enzyme-linked immunosorbent assay. Inflammatory cell recruitment sequence investigated using a monocytic line (THP-1) that can be activated into macrophage-like cells. adhesion THP-1 endothelial cells (human umbilical vein cells) studied fluorescent THP-1, their migration Boyden chambers, activation proliferation of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, whereas stem chambers immunofluorescence reverse transcription polymerase chain reaction characterization. Results Interleukin 6 decreased BRCS, it increased PCS. Transforming 1 significantly only BRCS. extracts did not affect cells, but inhibited migration. Moreover, lesser extent Finally, fibroblast proliferation without affecting By contrast, PCS Conclusions work shows sealers modulate potentials in vitro. It demonstrates has anti-inflammatory effects potential promote regeneration.