Identification of the Main Regulator Responsible for Synthesis of the Typical Yellow Pigment Produced by Trichoderma reesei.

摘要: ABSTRACT The industrially used ascomycete Trichoderma reesei secretes a typical yellow pigment during cultivation, while other species do not. A comparative genomic analysis suggested that putative secondary metabolism cluster, containing two polyketide-synthase encoding genes, is responsible for the synthesis. This cluster conserved in set of rather distantly related fungi, including Acremonium chrysogenum and Penicillium chrysogenum. In an attempt to silence T. reesei, genes transcription factors were individually deleted. For complete genetic proof-of-function, reinserted into genomes respective deletion strains. first factor (termed regulator 1 [Ypr1]) resulted full abolishment formation expression most this cluster. high-pressure liquid chromatography (HPLC) supernatants ypr1 its parent strain presence several compounds are all derived from same subsequent gas chromatography/mass spectrometry strongly indicated sorbicillin major HPLC peak. second factor, termed 2 (Ypr2), reduces gene activator Ypr1. IMPORTANCETrichoderma industry-scale production carbohydrate-active enzymes. During growth, it pigment. not favorable industrial enzyme because makes downstream process more complicated thus increases operating costs. study, we demonstrate which regulators influence synthesis Based on these data, also provide indication as under control finally biosynthesis These organized found relevant such antibiotic producers targeted manipulation important option any biotechnologically applied microorganism.