Candidate Antigens for Q Fever Serodiagnosis Revealed by Immunoscreening of a Coxiella burnetii Protein Microarray
作者： Paul A. Beare , Chen Chen , Timo Bouman , Jozelyn Pablo , Berkay Unal
DOI: 10.1128/CVI.00300-08
关键词: Serology 、 Protein microarray 、 Q fever 、 Coxiella burnetii 、 Immunoscreening 、 Virology 、 Biology 、 Context (language use) 、 Antibody 、 Antigen
摘要: Q fever is a widespread zoonosis caused by Coxiella burnetii. Diagnosis of usually based on serological testing patient serum. The diagnostic antigen test kits formalin-fixed phase I and II organisms the Nine Mile reference strain. Deficiencies this include (i) potential for cross-reactivity with other pathogens; (ii) an inability to distinguish between C. burnetii strains; (iii) need propagate purify burnetii, difficult potentially hazardous process. Consequently, there sensitive specific serodiagnostic tests utilizing defined antigens, such as recombinant protein(s). Here we describe use protein microarray comprehensively identify immunodominant antigens recognized antibody in context human infection or vaccination. Transcriptionally active PCR products corresponding 1,988 open reading frames (ORFs) were generated. Full-length proteins successfully synthesized from 75% ORFs using Escherichia coli-based vitro transcription translation system (IVTT). Nitrocellulose microarrays spotted crude IVTT lysates probed sera acute patients individuals vaccinated Q-Vax. Immune strongly reacted approximately 50 proteins, including previously identified immunogens, ankyrin repeat-domain containing protein, multiple hypothetical proteins. Recombinant selected array-reactive was generated, immunoreactivity confirmed enzyme-linked immunosorbent assay. This high-throughput method identifying immunoreactive will aid development antigen.
sci-hub.se 参考文章(33)
Jeffrey G. Shannon, Robert A. Heinzen, Infection of human monocyte-derived macrophages with Coxiella burnetii. Methods of Molecular Biology. ,vol. 431, pp. 189- 200 ,(2008) , 10.1007/978-1-60327-032-8_15
L R Hendrix, L P Mallavia, J E Samuel, Cloning and sequencing of Coxiella burnetii outer membrane protein gene com1. Infection and Immunity. ,vol. 61, pp. 470- 477 ,(1993) , 10.1128/IAI.61.2.470-477.1993
B. P. Marmion, R. A. Ormsbee, M. Kyrkou, J. Wright, D. A. Worswick, A. A. Izzo, A. Esterman, B. Feery, R. A. Shapiro, Vaccine prophylaxis of abattoir-associated Q fever: eight years' experience in Australian abattoirs. Epidemiology and Infection. ,vol. 104, pp. 275- 287 ,(1990) , 10.1017/S0950268800059458
W. Baehr, Y. X. Zhang, T. Joseph, H. Su, F. E. Nano, K. D. Everett, H. D. Caldwell, Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 85, pp. 4000- 4004 ,(1988) , 10.1073/PNAS.85.11.4000
X. Pan, A. Luhrmann, A. Satoh, M. A. Laskowski-Arce, C. R. Roy, Ankyrin repeat proteins comprise a diverse family of bacterial type IV effectors. Science. ,vol. 320, pp. 1651- 1654 ,(2008) , 10.1126/SCIENCE.1158160
P. Voland, D. L. Weeks, D. Vaira, C. Prinz, G. Sachs, Specific identification of three low molecular weight membrane-associated antigens of Helicobacter pylori. Alimentary Pharmacology & Therapeutics. ,vol. 16, pp. 533- 544 ,(2002) , 10.1046/J.1365-2036.2002.01221.X
Guoquan Zhang, James E Samuel, Vaccines against Coxiella infection. Expert Review of Vaccines. ,vol. 3, pp. 577- 584 ,(2004) , 10.1586/14760584.3.5.577
David P Regis, Carlota Dobaño, Paola Quiñones-Olson, Xiaowu Liang, Norma L Graber, Maureen E Stefaniak, Joseph J Campo, Daniel J Carucci, David A Roth, Huaping He, Philip L Felgner, Denise L Doolan, Transcriptionally active PCR for antigen identification and vaccine development: in vitro genome-wide screening and in vivo immunogenicity Molecular and Biochemical Parasitology. ,vol. 158, pp. 32- 45 ,(2008) , 10.1016/J.MOLBIOPARA.2007.11.009
Diane C. Cockrell, Paul A. Beare, Elizabeth R. Fischer, Dale Howe, Robert. A. Heinzen, A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells. Journal of Microbiological Methods. ,vol. 72, pp. 321- 325 ,(2008) , 10.1016/J.MIMET.2007.12.015
Bernard La Scola, Current laboratory diagnosis of Q fever Seminars in Pediatric Infectious Diseases. ,vol. 13, pp. 257- 262 ,(2002) , 10.1053/SPID.2002.127199