A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells.
作者： Diane C. Cockrell , Paul A. Beare , Elizabeth R. Fischer , Dale Howe , Robert. A. Heinzen
DOI: 10.1016/J.MIMET.2007.12.015
关键词:
摘要: Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. from The purity, yield, infectivity digitonin-prepped organisms are comparable that purified using cell by sonication.
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