Development of an enzyme-based assay for acetaminophen
作者： Peter M. Hammond , Michael D. Scawen , Tony Atkinson , R.Stewart Campbell , Christopher P. Price
DOI: 10.1016/0003-2697(84)90570-0
关键词: Enzymatic hydrolysis 、 Enzyme 、 Acetaminophen 、 Reaction conditions 、 Small sample 、 Chemistry 、 Chromatography 、 Biophysics 、 Cell biology 、 Biochemistry 、 Molecular biology
摘要: A new and novel method for determination of serum acetaminophen is described. The assay, which can be completed in less than 5 min, based on the enzymatic hydrolysis acetaminophen, with subsequent colorimetric detection aminophenol so produced. Various possible means estimation are described; final reaction conditions have been optimized maximum sensitivity assay speed. This compares favorably other available procedures; it requires only small sample volumes; rapid, simple, highly specific parent drug; neither great technical ability nor expensive instrumentation.
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