Frequent expression of genes for receptor tyrosine kinases and their ligands in human pancreatic cancer cells
作者： Eizo Kaneko , Tetsuro Oikawa , Akira Kono , Jiro Hitomi , Ken Yamaguchi
DOI: 10.1007/BF02825417
关键词: Platelet-derived growth factor receptor 、 Receptor tyrosine kinase 、 Cancer research 、 Growth factor receptor inhibitor 、 Hepatocyte growth factor 、 Growth factor receptor 、 Transforming growth factor 、 Biology 、 Amphiregulin 、 Pancreatic cancer
摘要: Limited information is available concerning the involvement of growth factor receptors and their ligands in pathogenesis human pancreatic cancer. We analyzed 12 cancer cell lines by Northern blot analysis for expression 9 receptor tyrosine kinases (RTKs) 6 factors. The effect a monoclonal antibody (MAb) against transforming factor-α (TGF-α) on vitro was also assessed. mRNA EGF-R, c-erbB-2 c-erbB-3 expressed (100%), 7 (58%), respectively, examined. In addition, 8 (67%) c-met/receptor hepatocyte factor. As ligands, TGF-α detected 10 (83%) lines; MAb inhibited 2 Furthermore, amphiregulin (AR) lines. Coexpression TGF-α, AR, EGF-R observed (75%) These results support concept that several specified types RTKs are closely involved regulation cells.
springer.com
sci-hub.se 参考文章(65)
A. Ciccodicola, R. Dono, S. Obici, A. Simeone, M. Zollo, M. G. Persico, Molecular characterization of a gene of the 'EGF family' expressed in undifferentiated human NTERA2 teratocarcinoma cells. The EMBO Journal. ,vol. 8, pp. 1987- 1991 ,(1989) , 10.1002/J.1460-2075.1989.TB03605.X
H Watanabe, R Manabe, S Kinoshita, C Yanaihara, Y Kinoshita, Y Ohashi, M Motokura, K Oshiden, T Mano, Presence of epidermal growth factor in human tears. Investigative Ophthalmology & Visual Science. ,vol. 30, pp. 1879- 1882 ,(1989)
Chan Am, King Hw, Deakin Ea, Cooper Cs, Tempest Pr, Brookes P, Primary structure of the met protein tyrosine kinase domain. Oncogene. ,vol. 1, pp. 229- 233 ,(1987)
D. F. Stern, M. P. Kamps, EGF-stimulated tyrosine phosphorylation of p185neu: a potential model for receptor interactions. The EMBO Journal. ,vol. 7, pp. 995- 1001 ,(1988) , 10.1002/J.1460-2075.1988.TB02906.X
F. H. Qiu, P. Ray, K. Brown, P. E. Barker, S. Jhanwar, F. H. Ruddle, P. Besmer, Primary structure of c-kit: relationship with the CSF-1/PDGF receptor kinase family-oncogenic activation of v-kit involves deletion of extracellular domain and C terminus The EMBO Journal. ,vol. 7, pp. 1003- 1011 ,(1988) , 10.1002/J.1460-2075.1988.TB02907.X
Jordan U. Gutterman, David V. Goeddel, Richard D. Williams, Axel Ullrich, Rik Derynck, Wendy H. Berger, Timothy S. Bringman, Synthesis of Messenger RNAs for Transforming Growth Factors α and β and the Epidermal Growth Factor Receptor by Human Tumors Cancer Research. ,vol. 47, pp. 707- 712 ,(1987)
Markus W. Büchler, Murray Korc, Martha E. Lopez, Gibbes R. Johnson, Michael S. Kobrin, Munehiro Yokoyama, Helmut Friess, Matthias Ebert, Induction and expression of amphiregulin in human pancreatic cancer Cancer Research. ,vol. 54, pp. 3959- 3962 ,(1994)
D.P. Hollywood, H.C. Hurst, A novel transcription factor, OB2‐1, is required for overexpression of the proto‐oncogene c‐erbB‐2 in mammary tumour lines. The EMBO Journal. ,vol. 12, pp. 2369- 2375 ,(1993) , 10.1002/J.1460-2075.1993.TB05891.X
C Birchmeier, D Birnbaum, G Waitches, O Fasano, M Wigler, Characterization of an activated human ros gene. Molecular and Cellular Biology. ,vol. 6, pp. 3109- 3116 ,(1986) , 10.1128/MCB.6.9.3109
D Martin-Zanca, R Oskam, G Mitra, T Copeland, M Barbacid, Molecular and biochemical characterization of the human trk proto-oncogene. Molecular and Cellular Biology. ,vol. 9, pp. 24- 33 ,(1989) , 10.1128/MCB.9.1.24