Ligand-induced transformation by a noninternalizing epidermal growth factor receptor
作者： A Wells , J. Welsh , C. Lazar , H. Wiley , G. Gill
关键词: Ligand (biochemistry) 、 Epidermal growth factor receptor 、 Growth factor receptor 、 Downregulation and upregulation 、 Biology 、 Cell biology 、 Biochemistry 、 Growth factor receptor inhibitor 、 Receptor 、 Epidermal growth factor 、 Internalization
摘要: Identification of a mutant epidermal growth factor (EGF) receptor that does not undergo downregulation has provided genetic probe to investigate the role internalization in ligand-induced mitogenesis. Contact-inhibited cells expressing this internalization-defective exhibited normal mitogenic response at significantly lower ligand concentrations than did wild-type receptors. A transformed phenotype and anchorage-independent were observed failed elicit these responses These findings imply activation protein tyrosine kinase activity cell membrane is sufficient for growth-enhancing effects EGF. Thus, can serve as an attenuation mechanism, without which transformation ensues.
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