Enzyme-aminoglycoside conjugates

摘要: Methods and compositions are provided for conjugating a wide variety of compounds, particularly polyfunctional having mercapto group, either naturally present or synthetically introduced, to polyamino compound, polypeptide (including proteins). The method employs haloalkyl-carbonyl which is conjugated one more the amino groups under mild acylating conditions. This followed by combining acylated compound with containing whereby halogen displaced sulfur group form stable thioether linkage. resulting conjugates, depending on compounds involved, can find uses in immunoassays, as hapten-antigen conjugates production antibodies, ligand analog enzyme use reagents controlling distribution substitution compound.