Protein-polysaccharide conjugate vaccines and other immunological reagents prepared using homobifunctional and heterobifunctional vinylsulfones, and processes for preparing the conjugates

摘要: A method is disclosed for producing a soluble conjugate vaccine, and preferably protein/polysaccharide conjugates. In this process, the polysaccharide reacted with reagent so as to provide functional group on molecule. Once in place, homobifunctional or heterobifunctional vinylsulfone produce derivatized polysaccharide. Thereafter, protein conjugate. If desired, may be prior conjugation reaction step. an alternative embodiment, functionalized reactive then protein. This Optionally, reaction. As another alternative, directly pendant of crosslinking agent. The coupled (which not functionalized). similar manner, agent molecule