MicroRNA 107 Partly Inhibits Endothelial Progenitor Cells Differentiation via HIF-1β
作者： Shu Meng , JiaTian Cao , LianSheng Wang , Qing Zhou , YiGang Li
DOI: 10.1371/JOURNAL.PONE.0040323
关键词: Small hairpin RNA 、 Cellular differentiation 、 microRNA 、 Cell biology 、 Progenitor cell 、 CD31 、 Regulation of gene expression 、 Downregulation and upregulation 、 Biology 、 Molecular biology 、 Stem cell 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Endothelial progenitor cells (EPCs) play an important role in tissue repair after ischemic heart disease. In particular, the recovery of endothelial function is reliant on ability and rate EPCs differentiate into mature cells. The present study evaluated effect microRNA 107 (miR-107) mechanism differentiation. were isolated from rats' bone marrow miR-107 expression hypoxic normoxic conditions measured by real-time qualitative PCR. CD31 was analyzed flow cytometry eNOS examined PCR western blotting these used as markers EPC order to reveal mechanism, we miR107 inhibitor lentiviral vector expressing a short hairpin RNA (shRNA) that targets hypoxia-inducible factor-1 β (HIF-1β) alter HIF-1β expression. MiR-107 increased under conditions. Up-regulation partly suppressed differentiation induced hypoxia, while down-regulation promoted target. This indicated up-regulated hypoxia prevent via its target HIF-1β. physiological mechanisms must be if it potential anti-ischemia therapeutic regime.
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