Genotyping and screening of reassortant live-attenuated influenza B vaccine strain
作者： Eun-Young Lee , Kwang-Hee Lee , Eun-Ju Jung , Yo Han Jang , Sang-Uk Seo
DOI: 10.1016/J.JVIROMET.2009.10.022
关键词: Attenuated vaccine 、 Reassortment 、 Genotyping 、 Virulence 、 Virus 、 Multiplex polymerase chain reaction 、 Orthomyxoviridae 、 Genetics 、 Biology 、 Virology 、 Influenzavirus B
摘要: Live-attenuated influenza virus vaccines can be generated by reassortment of gene segments between an attenuated donor strain and a virulent wild-type virus. The annual production schedule for the seasonal vaccine necessitates rapid efficient genotyping reassorted progeny to identify desired strains. This study describes multiplex RT-PCR system capable identifying each segment from cold-adapted virus, B/Lee/40ca. specificity amplification was optimized testing various B viruses. resulting method is sensitive enough routine identification reassortant clones constellation, consisting six H N genes By providing more means candidate strains, this could implemented expedite generation component allow time culture process final vaccine.
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