AP-ECD methods and apparatus for mass spectrometric analysis of peptides and proteins

摘要: An in-source atmospheric pressure electron capture dissociation (AP-ECD) method and apparatus for mass spectrometric analysis of peptides proteins. electrified sprayer generates a multiply-charged peptide/protein ions from sample solution, source electrons negative reagents, flow gas guiding positively charged the to downstream reaction region within guide. The being at or near substantially free electric field sprayer. In another embodiment, uses transfer (ETD), in event that anions are substituted as reagents. Fragment exiting subsequently passed into analyzer spectrometer ions.