Involvement of an Orphan Transporter, SLC22A18, in Cell Growth and Drug Resistance of Human Breast Cancer MCF7 Cells.
作者： Shingo Ito , Yu Fujino , Seiryo Ogata , Mio Hirayama-Kurogi , Sumio Ohtsuki
DOI: 10.1016/J.XPHS.2018.08.011
关键词: Cancer 、 Cell 、 Vinca alkaloid 、 Intracellular organelle 、 Cell growth 、 Tumor suppressor gene 、 Cancer research 、 CD44 、 Biology 、 Microtubule polymerization
摘要: The SLC22A18 gene, which encodes an orphan transporter, is located at the 11p15.5 imprinted region, important tumor suppressor gene region. However, role of in suppression remains unclear. Here, we investigated involvement cell growth, invasion, and drug resistance MCF7 human breast cancer line. Western blot analysis indicated that predominantly expressed intracellular organelle membranes. Quantitative proteomics showed knockdown significantly altered expression 578 (31.0%) 1867 proteins identified, including related to malignancy poor prognosis cancer. (1) increased growth concomitantly with a >7-fold increase annexin A8 (involved migration; predictor prognosis), (2) induced spherical morphology cells nearly 3-fold CD44 regulation malignant phenotypes), (3) chemosensitivity vinca alkaloids >80% reduction doublecortin-like kinase 1 microtubule polymerization). Our results suggest may act as by regulating levels growth-related proteins, might show therapeutic efficacy against low-SLC22A18-expressing
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