Changes in interfacial potentials induced by carbonylcyanide phenylhydrazone uncouplers: possible role in inhibition of mitochondrial oxygen consumption and other transport processes.
作者： Juan Reyes , Dale J. Benos
DOI: 10.3109/09687688409150281
关键词: Oxygen 、 Rotenone 、 Potential change 、 Phosphatidylcholine 、 Membrane potential 、 Respiratory chain 、 Stereochemistry 、 Membrane 、 Chemistry 、 Mitochondrion 、 Biophysics
摘要: The charged and uncharged forms of carbonyl-cyanide phenylhydrazone uncouplers bind to phosphatidylcholine monolayers in a dose-dependent fashion, inducing changes the interfacial potential these model membranes. change produced by un-coupler is composed double-layer an internal electrostatic (boundary and/or dipole). Changes induced mitochondrial membranes can explain both inhibition oxygen consumption (QO2) caused competition shown succinate when mitochondria are respiring presence rotenone. From results from dose-response curves QO2 versus uncoupler concentrations, we conclude that 1 μM upper limit for free concentration medium avoid unwanted side effects during cell physiology studies require total uncoupling.
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