High diversity of invasive Haemophilus influenzae isolates in France and the emergence of resistance to third generation cephalosporins by alteration of ftsI gene.

摘要: Abstract Background Invasive infections due to Haemophilus influenzae are infrequent following the implementation of vaccination against H. serotype b. However, their changing epidemiology may not be clear a lack appropriate genotyping methods combined with antibiotic susceptibility analyses which do discriminate invasive and non-invasive isolates. We aimed describe recent epidemiological trends in France explore microbiological characteristics versus Methods All culture- PCR-confirmed cases isolated from sterile site, that were received at French national reference centre for during year 2017 (n = 138) characterized by whole genome sequencing (WGS), serotyping testing. also included 100 isolates infections. Findings Most non-typeable (99%) this proportion was significantly less among 75%, p  Interpretation Our data suggest differed phenotypically genotypically The high ampicillin resistance mutation ftsI biological cost these mutations on function PBP3 can lead lower bacterial invasiveness. WGS should used routinely characterization order reliably follow emergence, spread mechanism resistance.