Increased exhaled nitric oxide precedes lung fibrosis in two murine models of systemic sclerosis.

摘要: Exhaled nitric oxide (NO) is increased as a result of lung inflammation, which in turn causes subsequent interstitial disease patients with systemic sclerosis (SSc). However, the exact time course inflammatory and fibrotic changes SSc has not yet been described. Our objective was to assess chronological evolution processes mice pre-treated hypochlorous acid (HOCl) or bleomycin. C57BL/6 were randomized into three groups receiving subcutaneous injections HOCl, bleomycin, PBS for 2, 4 6 weeks. NO (eNO) measured at end each injection period after 2 resting weeks without (8 week group). Mice then sacrificed obtain skin tissues measure synthases (NOS) expression. Increased eNO, inducible NOS nitrotyrosine expression bronchial epithelium, neutrophils macrophages observed early phases both HOCl- bleomycin-treated mice. Conversely, vascular endothelial decreased significantly 6th 8th Skin fibrosis from 4th week. We conclude that inflammation occurs injury reflected by exhaled expression, precedes lungs bleomycin HOCl. Early detection treatment pulmonary might be useful preventing occurrence patients.