Homozygous loss of the cyclin-dependent kinase 4-inhibitor (p16) gene in human leukemias

作者: S Ogawa , N Hirano , N Sato , T Takahashi , A Hangaishi

DOI: 10.1182/BLOOD.V84.8.2431.2431

关键词: Chromosome 9Cyclin-dependent kinaseLeukemiaAlleleCancer researchGeneABLBiologyMolecular biologyCyclin-dependent kinase 4Acute lymphocytic leukemia

摘要: Recently, it has been shown that the homozygous deletion of cyclin-dependent kinase-4 inhibitor (CDK4I;p16) gene, which is mapped to chromosome 9p21, frequently observed in a wide spectrum human cancers, including leukemias. Therefore, CDK4I gene thought be putative tumor-suppressor gene. We report here both alleles were completely or partially deleted leukemia cells derived from patients and established cell lines. Thirty-seven hematopoietic lines samples 72 with leukemias examined for loss locus by Southern blot analysis. found part whole was homozygously 14 37 (38%) 4 (6%) patients, 45 acute myelocytic leukemia, lymphocytic (ALL), 13 chronic blastic crisis. In lines, detected variety lineages, whereas all cases showing confined ALL. It should noted 2 them had no cytogenetic abnormalities 9. Our results suggest function may contribute immortalization play causative role at least development

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