Ochratoxin A detection method based on energy transfer between two types of quantum dots
作者： Jiang Hu , Xiong Yonghua , Xu Yang , Xu Hengyi , Guo Liang
DOI:
关键词: Optoelectronics 、 Quantum dot 、 Quantum 、 Fluorescence 、 Conjugate 、 Single-domain antibody 、 Resonance 、 Field (physics) 、 Ochratoxin A 、 Materials science
摘要: The present invention belongs to the field of analysis detection, and discloses an ochratoxin A detection method based on energy transfer between two types quantum dots. According method, green dots are selected conjugate with OTA, red single domain antibody anti-OTA, obtained materials mixed, distance is reduced due specific binding antigen produce resonance so as cause fluorescent dot fluorescence enhanced fluorescence, when reaction system contains free OTA concurrently competes against dot-conjugated in antibody, concentration directly affects efficiency dots, logarithm form proportion relationship; has characteristics short time, high sensitivity, accuracy, simple operation process, low cost like.
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