Survival and fertility of boar spermatozoa after freeze-thawing in extender supplemented with butylated hydroxytoluene.

摘要: ABSTRACT: This study evaluated the protective effect of butylated hydroxytoluene (BHT), a lipid-soluble antioxidant, against cryopreservation injuries to boar spermatozoa. In experiment 1, lowest BHT concentrations able reduce lipid peroxidation in spermatozoa were determined. Nine (ranging from 0.025 3.2 mM) evaluated, and (P < .05) production malondialdehyde (MDA), as an indicator peroxidation, was obtained when ranged 0.2 1.6 mM. 2, sperm survivability added postthaw freezing extender by measuring degree (using MDA production) parameter such motility, plasma membrane acrosome integrity, cell apoptosis. The ability thawed fertilize vitro—matured oocytes embryos develop blastocyst stage vitro also assessed. Pooled sperm-rich fractions collected 3 mature Pietrain boars frozen 0.5-mL straws after dilution with lactose-egg yolk-glycerol-Orvus ES Paste supplemented 0, 0.2, 0.4, 0.8, mM BHT. Postthaw survival, 30 150 minutes thawing, higher BHT-treated spermatozoa, being significant 0.8 addition resulted decrease concentration irrespective level used. had no on oocyte cleavage rates, but development improved for derived 0.4 (16% vs 29% blastocysts per total oocytes; P .05). conclusion, under conditions tested present study, overall efficiency