In Vitro Selection of Diversely Functionalized Aptamers.
作者： Dehui Kong , Wayland Yeung , Ryan Hili
DOI: 10.1021/JACS.7B07241
关键词: DNA 、 Oligonucleotide 、 Thrombin 、 Nucleic acid 、 Polymerization 、 Dissociation constant 、 Combinatorial chemistry 、 Aptamer 、 Chemistry 、 Biochemistry 、 Genomic library
摘要: We describe the application of T4 DNA ligase-catalyzed templated oligonucleotide polymerization toward evolution a diversely functionalized nucleic acid aptamer for human α-thrombin. Using 256-membered ANNNN comonomer library comprising 16 sublibraries modified with different functional groups, highly thrombin was raised dissociation constant 1.6 nM. The found to be selective and required modifications binding affinity. This study demonstrates most differentially discovered by in vitro selection should enable future exploration group dependence during polymer activity.
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