Functional expression of voltage-gated sodium channels in primary cultures of human cervical cancer.

摘要: Cervical cancer (CaC) is the third most frequent cause of death from among women in world and first females developing countries. Several ion channels are upregulated cancer, actually potassium have been suggested as tumor markers therapeutic targets for CaC. Voltage-gated sodium (VGSC) activity involved proliferation, motility, invasion prostate breast cells; however, participation this type CaC has not explored. In present study, we identified both at molecular electrophysiological level VGSC primary cultures human cervical carcinoma biopsies. With whole cell patch clamp technique, isolated a voltage-gated Na(+) current main component inward all investigated cells. Sodium was characterized by its kinetics, voltage dependence, sensitivity to tetrodotoxin (TTX) block dependence [Na(+)](o). By analyzing expression mRNAs encoding TTX-sensitive channel alpha subunits with standard RT-PCR specific primers, detected Na(v)1.2, Na(v)1.4, Na(v)1.6, Na(v)1.7 transcripts total RNA obtained biopsies Restriction enzyme analysis PCR products consistent nature corresponding genes. Notably, only Na(v)1.4 were normal cervix. The results show time functional CaC, suggest that these might be considered potential cancer.