Isolation, culture, and regeneration of moth bean Vigna aconitofolia leaf protoplasts
作者： N.S. Shekhawat , A.W. Galston
DOI: 10.1016/0304-4211(83)90097-4
关键词: Biology 、 Asparagine 、 Somatic embryogenesis 、 Protoplast 、 Botany 、 Drought tolerance 、 Plating efficiency 、 Vigna 、 Vigna aconitifolia 、 Auxin
摘要: Abstract Mesophyll protoplasts, isolated with purified Driselase from a drought resistant food crop (moth bean, Vigna aconitifolia ), were cultured in standing microdrops. High division rates and plating efficiency obtained by purifying the enzyme, adding glutamine asparagine to culture medium, gradually reducing levels of osmiticum. On auxin free protoplast-derived calli produced embryoids which developed into complete plants.
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