A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages.
作者： Chandrayana Mukherjee , Christine Hale , Subhankar Mukhopadhyay
DOI: 10.1007/978-1-4939-7837-3_2
关键词: Myeloid 、 Cell culture 、 Cell biology 、 Inflammation 、 Induced pluripotent stem cell 、 Chemistry 、 Human Induced Pluripotent Stem Cells 、 Innate immune system 、 Embryoid body
摘要: Macrophages differentiated from human induced pluripotent stem cells (hiPSCs) provide an alternative new tool overcoming some of the limitations existing models for macrophages, such as macrophage-like cell lines and primary monocyte-derived macrophages. A combination different cytokines growth factors can differentiate hiPSCs toward myeloid lineage. Here we describe a simple multistep protocol differentiating into functional This includes derivation three germ-line containing embryoid bodies (EBs) iPSCs, generation precursors EBs, finally maturation Technical procedure specific culture conditions associated with each these steps are discussed in detail.
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