Treatment of experimental glioma by administration of adenoviral vectors expressing Fas ligand.

摘要: Fas ligand (FasL) is a cytokine, produced by activated T cells and NK cells, that triggers apoptosis of Fas-positive target including human glioma cells. As shown here, in vitro infection rat F98 LN18 cell lines with recombinant adenovirus (rAd) expressing FasL cDNA under control the cytomegalovirus promoter (rAd-CMV-FasL) induced striking cytotoxicity but not Fas-negative subline F98/ZH. The extent FasL-mediated cytotoxic effects outranged expectations based on expression beta-galactosidase (beta-Gal) infected virus lacZ gene (rAd-CMV-lacZ). detection bioactivity supernatants provides evidence bystander mechanism involving action uninfected In tumor-bearing rats, rAd-CMV-FasL increased mean survival time 50% compared rAd-CMV-lacZ or untreated controls. These ...