Detection of genomic DNA methylation with denaturing high performance liquid chromatography.
作者： Romaica A OMARUDDIN , M Ahmad CHAUDHRY
DOI: 10.1111/J.1749-0774.2010.00084.X
关键词: DNA methylation 、 Bisulfite sequencing 、 RNA-Directed DNA Methylation 、 Epigenomics 、 Biology 、 Genetics 、 Molecular biology 、 Methylated DNA immunoprecipitation 、 Illumina Methylation Assay 、 Differentially methylated regions 、 DNA methyltransferase
摘要: DNA methylation contributes to the epigenetic control of gene expression. Variations in status can result silencing genes. methyltransferase converts cytosine 5-methyl CpG islands located promoter regions When are hypermethylated, is repressed/silenced, and similarly when it hypomethylated, transcription take place expressed. The classical methods detect require labor-intensive time-consuming steps. As a large-scale expression profiling studies, high-throughput techniques needed screen for alterations patterns. Denaturing high performance liquid chromatography (DHPLC) reliable, highly sensitive technique mutation discovery. In present study we examined suitability DHPLC technology pattern several We report reliable reproducible results distinguishing methylated unmethylated human PCDHGB6, c-MYC, MGMT1, CDKN2A/p16, ATM These profiles were independently confirmed with bisulfite genomic sequencing. conclusion, serves as rapid screening tool monitor could be used increase throughput efficiency analysis.
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