DNA methylation of multiple promoter-associated CpG islands in adult acute lymphocytic leukemia.

作者: Steven M. Kornblau , Guillermo Garcia-Manero , Hagop M. Kantarjian , Terry L. Smith , Jerry Daniel

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摘要: Purpose: Aberrant methylation of promoter-associated CpG islands is an epigenetic oncogenic mechanism. The objective this study was to define the characteristics patients with acute lymphocytic leukemia (ALL). Experimental Design: Using bisulfite-PCR followed by restriction enzyme digestion (COBRA), we have analyzed status 10 in 80 untreated adult ALL. Results: Mean density MDR1, THBS2, MYF3, ER, p15, THBS1, CD10, C-ABL , and p16 24.5%, 20.8%, 17.6%, 16.1%, 11.3%, 8.9%, 4.5%, 3.7%, 1.3% respectively. p73 methylated 17 cases (21.2%). A total 86.2% had at least one gene, 42.5% three or more genes. MDR1 inversely correlated age ( P = 0.01). CD10 expression 0.0001). Methylation THBS1 associated presence Philadelphia chromosome, whereas p210 variant chromosome. In univariate analysis, a favorable outcome 0.02), p73, p15 trend toward worse prognosis. Conclusions: DNA very common ALL potentially defines subgroups distinct clinical biological characteristics.

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