In vitro examination of DHA-edible micro algae

作者: C. Boeckaert , B. Vlaeminck , J. Mestdagh , V. Fievez

DOI: 10.1016/J.ANIFEEDSCI.2006.08.015

关键词: Linolenic acidFish oilLinseed oilConjugated linoleic acidLinoleic acidSunflower oilDocosahexaenoic acidVaccenic acidBiologyFood scienceBiochemistry

摘要: Abstract Effects of a docosahexaenoic acid (DHA)-edible algae product, Aquagrow-DHA, on lipolysis and biohydrogenation linoleic (C18:2 n-6) was studied in 24 h vitro incubations with buffer (20 ml), sheep rumen contents (5 ml), hay (0.4 g) sunflower oil (20 mg). Four Aquagrow-DHA levels (0, 20.8, 41.6, 83.3 mg) were tested addition lard ensured that the total fat content constant. Increasing proportionally inhibited hydrogenation C18:2 n-6, provoking an accumulation C18:1 trans fatty acids (mainly t10 + t11). Analogous for 6 linseed (20 mg) instead oil, resulted t11c15 and, to lower extent, t10 + t11 showed reduced rate linolenic (C18:3 n-3). Comparison these results individual constituents whole biomass revealed observed effects related presence DHA. Finally, under 50:50 H2:CO2 atmosphere suggested direct inhibitory effect unesterified DHA hydrogenating bacteria involved conversion isomers, rather than H2 availability.

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