The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia.
作者： Bernhard Setzer , Maria Bächle , Marc C. Metzger , Ralf J. Kohal
DOI: 10.1016/J.BIOMATERIALS.2008.10.054
关键词: Analytical chemistry 、 Cytoskeleton organization 、 Cubic zirconia 、 Materials science 、 Integrin 、 Cellular differentiation 、 Bone morphogenetic protein 7 、 Scanning electron microscope 、 Biophysics 、 RUNX2 、 Titanium
摘要: Abstract The osteoblastic cell-line hFOB 1.19 with the potential to proliferate and differentiate revealed that cellular differentiation is not affected by material roughness on newly developed zirconia implant materials. Materials under investigation were surfaces machined titanium (Ti-m), modified (TiUnite ® ), (TZP-A-m), (ZiUnite alumina-toughened (ATZ-m) (ATZ-mod). After surface description scanning electron microscopy (SEM) atomic force (AFM), proliferation (EZ4U, Casy1) examined after days 1, 3, 7, 14, 21, 28. Osteogenic was visualized alkaline phosphatase staining, mineralization assay (alizarin red) expression analysis (RT-PCR) of bone- extracellular matrix-related genes. Proliferation rough reduced both zirconia. Cell-attachment cytoskeleton organization documented confocal laser (CLSM) elucidated attenuated cell attachment within first 4 h be reason for impaired proliferation. A specific up-regulation m-RNAs in an early event (RUNX2, NELL-1, RUNX3, BMP7) a late (Integrin B3) could observed TiUnite ZiUnite . For IBSP Integrin B1 described at day 21. In total, neither nor roughness.
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sci-hub.se 参考文章(42)
D M Brunette, The effects of implant surface topography on the behavior of cells. International Journal of Oral & Maxillofacial Implants. ,vol. 3, pp. 231- 246 ,(1988)
Denise Seabold, Zakiah M. Isa, Galen B. Schneider, Clark M. Stanford, Rebecca Zaharias, Effects of fluoride-modified titanium surfaces on osteoblast proliferation and gene expression. International Journal of Oral & Maxillofacial Implants. ,vol. 21, pp. 203- 211 ,(2006)
Benjamin Geiger, Alexander Bershadsky, Roumen Pankov, Kenneth M. Yamada, Transmembrane crosstalk between the extracellular matrix and the cytoskeleton Nature Reviews Molecular Cell Biology. ,vol. 2, pp. 793- 805 ,(2001) , 10.1038/35099066
Frederick Grinnell, Cellular adhesiveness and extracellular substrata. International Review of Cytology-a Survey of Cell Biology. ,vol. 53, pp. 65- 144 ,(1978) , 10.1016/S0074-7696(08)62241-X
R.S. Tuan, B. Groessner-Schreiber, Enhanced extracellular matrix production and mineralization by osteoblasts cultured on titanium surfaces in vitro. Journal of Cell Science. ,vol. 101, pp. 209- 217 ,(1992) , 10.1242/JCS.101.1.209
M. Subramaniam, Syed M. Jalal, David J. Rickard, Steven A. Harris, Mark E. Bolander, Thomas C. Spelsberg, Further characterization of human fetal osteoblastic hFOB 1.19 and hFOB/ER? cells: Bone formation in vivo and karyotype analysis using multicolor fluorescent in situ hybridization Journal of Cellular Biochemistry. ,vol. 87, pp. 9- 15 ,(2002) , 10.1002/JCB.10259
Tobias P. Kunzler, Tanja Drobek, Martin Schuler, Nicholas D. Spencer, Systematic study of osteoblast and fibroblast response to roughness by means of surface-morphology gradients Biomaterials. ,vol. 28, pp. 2175- 2182 ,(2007) , 10.1016/J.BIOMATERIALS.2007.01.019
Frank Lüthen, Regina Lange, Petra Becker, Joachim Rychly, Ulrich Beck, J.G. Barbara Nebe, The influence of surface roughness of titanium on β1- and β3-integrin adhesion and the organization of fibronectin in human osteoblastic cells Biomaterials. ,vol. 26, pp. 2423- 2440 ,(2005) , 10.1016/J.BIOMATERIALS.2004.07.054
Gary S Stein, Jane B Lian, Andre J Van Wijnen, Janet L Stein, Martin Montecino, Amjad Javed, Sayyed K Zaidi, Daniel W Young, Je-Yong Choi, Shirwin M Pockwinse, None, Runx2 control of organization, assembly and activity of the regulatory machinery for skeletal gene expression. Oncogene. ,vol. 23, pp. 4315- 4329 ,(2004) , 10.1038/SJ.ONC.1207676
Xiaomei Liu, Jung Yul Lim, Henry J. Donahue, Ravi Dhurjati, Andrea M. Mastro, Erwin A. Vogler, Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro ☆ Biomaterials. ,vol. 28, pp. 4535- 4550 ,(2007) , 10.1016/J.BIOMATERIALS.2007.06.016