DNA bioprints: simple nonisotopic DNA fingerprints with biotinylated probes.
作者： Sérgio D. J. Pena , Andrea M. Macedo , Nelder F. Gontijo , Arnaldo M. Medeiros , Julio Cesar C. Ribeiro
关键词: DNA 、 Computational biology 、 Oligonucleotide 、 Biotinylation 、 Recombinant DNA 、 DNA profiling 、 Agarose 、 Molecular biology 、 Molecular probe 、 Southern blot 、 Biology
摘要: The discovery of multilocal DNA fingerprinting represented a revolution in criminal identification and paternity testing. However, for routine use clinical laboratories, the standard fingerprint methodology is too complex. We have been successful development simplified nonisotopic system using biotin-labeled probes which we called bioprints. To achieve this explored three main technical features: utilization biotinylated nonradioactive as simpler substitute 32P-labeled probes, oligonucleotide recombinant direct hybridization dried agarose gel Southern blots. In article review our results
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