Successful extraction of DNA from 100‐year‐old herbarium specimens of the liverwort Bazzania trilobata
作者： Kamila Jankowiak , Katarzyna Buczkowska , Zofia Szweykowska-Kulinska
DOI: 10.2307/25065361
关键词: Herbarium 、 DNA 、 Biology 、 Material storage 、 Botany 、 Bazzania trilobata 、 Extraction (chemistry) 、 DNA extraction
摘要: Using a modified DNeasy Plant Mini Kit method to extract DNA, we were able isolate DNA from 100-year-old liverwort herbarium specimens. We sucessfully isolated 18 specimens of the Bazzania trilobata collected during 20 t h century. Our observations suggest that, at least over 100 years material storage, most crucial factor in successful isolation is drying, not age sample.
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