The contribution of in vitro technology and cryogenic storage to conservation of indigenous plants

作者: Eric Bunn , Shane Turner , Maggie Panaia , Kingsley W. Dixon

DOI: 10.1071/BT06065

关键词: BiologyPlant ecologyThreatened speciesIndigenousGermplasmEx situ conservationSomatic embryogenesisPaleobotanyPlant speciesBotany

摘要: In vitro culture has enabled a variety of recalcitrant and threatened plant taxa to be micropropagated in the absence viable conventional propagation methods. Cryogenic storage research provided alternative protocols for efficient long-term germplasm many species. Recent advances tissue-culture methods such as somatic embryogenesis have production >20 000 embryos native Australian rush few months, far higher than other these types plants. are reported >30 species vascular plants, seed numerous mycorrhizal fungi (mainly orchid spp.), greatly extending range type material that can stored through application cryogenic The role initiatives botanic gardens biodiversity conservation restoration is discussed, using examples successful ex situ cryogenic-storage research.

参考文章(48)
D.J. Growns, C. Newell, J.A. Considine, G. Yan, Waxflower selection, breeding and development - an overview. IV International Symposium on New Floricultural Crops, Chania, Crete, Greece, 22-27 May, 1999.. pp. 119- 124 ,(2000) , 10.17660/ACTAHORTIC.2000.541.17
A. Sakai, Cryopreservation of Germplasm of Woody Plants Springer, Berlin, Heidelberg. pp. 113- 129 ,(1986) , 10.1007/978-3-642-70576-2_8
Florent Engelmann, Importance of cryopreservation for the conservation of plant genetic resources. Cryopreservation of tropical plant germplasm: current research progress and application. Proceedings of an international workshop, Tsukuba, Japan, October, 1998. pp. 8- 20 ,(2000)
J.A. McComb, C. Tonkin, I.J. Bennett, In vitro propagation of Eucalyptus species McComb, J.A. <http://researchrepository.murdoch.edu.au/view/author/McComb, Jen.html>, Bennett, I.J. and Tonkin, C. (1996) In vitro propagation of Eucalyptus species. In: Taji, A. and Williams, R., (eds.) Tissue culture of Australian plants. University of New England, Armidale, NSW, Australia, pp. 112-156.. ,(1996)
M. P. Watt, F. C. Blakeway, R. Termignoni, S. M. Jain, Somatic Embryogenesis in Eucalyptus grandis and E. dunni Springer, Dordrecht. pp. 63- 78 ,(1999) , 10.1007/978-94-011-4774-3_4
M. Panaia, T. Senaratna, E. Bunn, K.W. Dixon, K. Sivasithamparam, Micropropagation of the critically endangered Western Australian species, Symonanthus bancroftii (F. Muell.) L. Haegi (Solanaceae) Plant Cell Tissue and Organ Culture. ,vol. 63, pp. 23- 29 ,(2000) , 10.1023/A:1006457624282
C.H. Bornman, Somatic seed in conifer biotechnology - a viable alternative to natural seed? South African Journal of Botany. ,vol. 68, pp. 119- 126 ,(2002) , 10.1016/S0254-6299(15)30411-7
Darren Touchell, S. R. Turner, T. Senaratna, E. Bunn, K. W. Dixon, Cryopreservation of Australian Species — The Role of Plant Growth Regulators Biotechnology in Agriculture and Forestry. pp. 373- 390 ,(2002) , 10.1007/978-3-662-04674-6_26
C. Thomson-Dans, A. Brown, N. Marchant, Western Australia's threatened flora. Department of Conservation and Land Management, Western Australia. ,(1998)