Synthetic octacalcium phosphate-enhanced reparative dentine formation via induction of odontoblast differentiation.

摘要: Synthetic octacalcium phosphate (OCP) has been suggested to be a useful biomaterial for the regeneration of hard tissues, including bone. However, it remains unknown whether OCP induces dentine formation by dental pulp. We investigated biomineralization pulp exposed synthetic in vitro and vivo. When was directly OCP, rapid reparative (RD) induced expression sialoprotein synthesis observed adjacent newly synthesized RD. inhibited proliferation rat cells also promoted their odontoblastic differentiation vitro, as alkaline phosphatase activity, mineralization level sialophosphoprotein were enhanced. Direct contact between is required exhibit its effects vitro. The Runx2, transcription factor whose downregulation closely related odontoblast differentiation, downregulated cultured with OCP. Structural changes during culture determined Fourier transform infrared spectroscopy. tended converted carbonate hydroxyapatite after incubation or without cells, which may analogous biological apatite crystals. Taken together, our data suggest that supports RD Runx2 involved stimulatory activity. Furthermore, OCP–apatite conversion this capacity Copyright © 2013 John Wiley & Sons, Ltd.