Inoculum Density-Dependent Mortality and Colonization of the Phyllosphere by Pseudomonas syringae.

作者: Mark Wilson , Steven E. Lindow

DOI: 10.1128/AEM.60.7.2232-2237.1994

关键词: ExtracellularHorticulturePseudomonadalesColonisationPseudomonadaceaeBotanyColonizationBacteriaPhyllosphereBiologyPseudomonas syringae

摘要: Pseudomonas syringae inocula containing cell concentrations ranging from 105 to 109 cells per ml were applied the primary leaves of bean plants. The plants incubated under conditions high temperature and illumination low relative humidity. Bacterial mortality rates proportional population decline inoculum lowest at highest concentrations. Addition a concentration heat-killed viable significantly reduced both rate cells. mechanisms underlying this density-dependent may include cooperative protective effects extracellular factors, such as bacterial polysaccharides, physical protection by neighboring Although epiphytic populations derived 108 or tended toward 106 CFU/g, presumed carrying capacity leaf, lower never achieved capacity. Assuming that P. reside in discrete protected sites, our results suggest concentrations, following period environmental stress, number have dropped zero some sites; hence, leaf could not be achieved.

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