Comparative ELISA reagents for detection of hepatitis B surface antigen (HBsAg).
作者： I. F. Camargo , A. M. C. Gaspar , C. F. T. Yoshida
DOI: 10.1590/S0074-02761987000200005
关键词: Antibody 、 Virology 、 Medicine 、 Antigen 、 Hepatitis b surface antigen 、 Reagent 、 HBsAg 、 Molecular biology 、 Horseradish peroxidase
摘要: Conjugates of goat anti-HBs IgG and horseradish peroxidase (HRP) prepared by two different methods, one using NaIO4 the other SPDP, were compared. Anti-HBs antibodies obtained from goat, rabbit guinea-pig tested as capture serum. The ELISA showed a sensitivity similar to RIA level antigen captation ranging 4.37 8.75 nanograms/ml was when or captures used combined with both SPDP conjugates.
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