Effects of temperature and aldehyde fixation on tissue water diffusion properties, studied in an erythrocyte ghost tissue model.

摘要: Ex vivo biological sample imaging can complement in MRI studies. Since ex studies are typically performed at room temperature, and samples frequently preserved by fixation, it is important to understand how environmental chemical changes dictated alter the physical MR properties of a sample. Diffusion relaxation time measurements were used assess effects temperature change aldehyde fixation on biophysical model tissue comprised erythrocyte ghosts suspended buffer or agarose gel. Sample was varied between 10°C 37°C. data analyzed with biophysically appropriate two-compartment exchange model. Temperature resulted complex alteration water diffusion due compartmental nature tissues membrane permeability. Formaldehyde, Karnovsky's solution, glutaraldehyde all caused statistically significant samples. Fixation large decreases proton T2, which restored near prefixation values washing free fixative from Water permeability also significantly altered fixation. This study demonstrates that relating chemically fixed requires an understanding preparation. Magn Reson Med, 2006. © 2006 Wiley-Liss, Inc.