Degradation potential of biological tissues fixed with various fixatives: an in vitro study.

摘要: The purpose of this study was to investigate the in vitro degradation potential porcine pericardia fixed with various aldehyde or epoxy compound (EC) fixatives, using bacterial collagenase and pronase. fixatives investigated were formaldehyde (FA), glutaraldehyde (GA), monofunctional EC (EX-131), multifunctional ECs (EX-810, EX-313, EX-512). Fresh pericardium used as a control. test samples well immersed 20-U/mL solution 10-U/ml pronase incubated at 37°C pH 7.5 for 24 h. extent each sample determined by measuring its increment free amino group content changes collagen structure, denaturation temperature, tensile stress after degradation. In general, tissue more notable than collagenase. As observed fresh tissue, EX-131 radically disintegrated either degradation, whereas other remained intact. reason may reside random molecular packing EC-fixed which led some loss helical integrity. This made penetration enzymes into biological easier. Of groups, tissues tetrafunctional (EX-521) trifunctional (EX-313) had relatively better resistance those bifunctional (EX-810). EX-313 EX-512 similar that FA- GA-fixed tissues. results indicated (EX-131) cannot resist However, (EX-313 EX-152)-fixed comparable (FA GA)-fixed Therefore, greater number functional groups should be chosen fixation increase enzymatic © 1997 John Wiley & Sons, Inc. J Biomed Mater Res, 35, 147–155,