The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia.

摘要: Human meniscus cells have a predominantly fibrogenic pattern of gene expression, but like chondrocytes they proliferate in monolayer culture and lose the expression type II collagen. We investigated potential human cells, which were expanded with or without fibroblast growth factor 2 (FGF2), to produce matrix three-dimensional cell aggregate cultures chondrogenic medium at low (5%) normal (20%) oxygen tension. The presence FGF2 during expansion enhanced re-expression collagen 200-fold subsequent cultures. This was increased further (400-fold) by 5% oxygen. Cell aggregates FGF2-expanded accumulated more proteoglycan (total glycosaminoglycan) over 14 days deposited II-rich matrix. matrix-associated proteoglycans (biglycan fibromodulin) also hypoxia. Meniscus after can therefore respond signals, this is tension