Matrix formation is enhanced in co-cultures of human meniscus cells with bone marrow stromal cells.
作者： Norah-Faye Matthies , Aillette Mulet-Sierra , Nadr M. Jomha , Adetola B. Adesida
DOI: 10.1002/TERM.1489
关键词: Chemistry 、 Cell biology 、 Oxygen tension 、 Type I collagen 、 Meniscus (anatomy) 、 Extracellular matrix 、 Biomedical engineering 、 Matrix (biology) 、 Tissue engineering 、 Bone marrow 、 Stromal cell
摘要: The ultimate aim of this study was to assess the feasibility using human bone marrow stromal cells (BMSCs) supplement meniscus for tissue engineering and regeneration. Human menisci were harvested from three patients undergoing total knee replacements. Meniscus released after collagenase treatment. BMSCs iliac crest expanded in culture until passage 2. Primary co-cultured vitro three-dimensional (3D) pellet at different cell–cell ratios 3 weeks under normal (21% O2) or low (3% oxygen tension presence serum-free chondrogenic medium. Pure pure cell pellets served as control groups. generated assessed biochemically, histochemically by quantitative RT–PCR. Co-cultures primary resulted with increased (1.3–1.7-fold) deposition proteoglycan (GAG) extracellular matrix (ECM) relative tissues derived alone 21% O2. GAG formation also enhanced (1.3–1.6-fold) 3% O2 conditions. Alcian blue staining confirmed GAG-rich matrix. mRNA expression type I collagen (COL1A2), II (COL2A1) aggrecan upregulated pellets. However, SOX9 HIF-1α not significantly modulated co-culture. Co-culture ECM formation. Co-delivery can, principle, be used regenerative medicine strategies repair defects. Copyright © 2012 John Wiley & Sons, Ltd.
sci-hub.se 参考文章(57)
Samuel Adams, Mark Randolph, Thomas Gill, Tissue engineering for meniscus repair. Journal of Knee Surgery. ,vol. 18, pp. 25- 30 ,(2010) , 10.1055/S-0030-1248154
W. G. Rodkey, Menaflex (TM) Collagen Meniscus Implant: Basic Science Springer, Berlin, Heidelberg. pp. 367- 371 ,(2010) , 10.1007/978-3-642-02450-4_46
P J Roughley, D McNicol, Extraction and characterization of proteoglycan from human meniscus. Biochemical Journal. ,vol. 185, pp. 705- 713 ,(1980) , 10.1042/BJ1850705
AdetolaB Adesida, LisaM Grady, WasimS Khan, TimothyE Hardingham, The matrix-forming phenotype of cultured human meniscus cells is enhanced after culture with fibroblast growth factor 2 and is further stimulated by hypoxia. Arthritis Research & Therapy. ,vol. 8, pp. 1- 9 ,(2006) , 10.1186/AR1929
Madhu M. Bhargava, Erik T. Attia, George A. C. Murrell, Martin M. Dolan, Russell F. Warren, Jo A. Hannafin, The Effect of Cytokines on the Proliferation and Migration of Bovine Meniscal Cells The American Journal of Sports Medicine. ,vol. 27, pp. 636- 643 ,(1999) , 10.1177/03635465990270051601
Clemente Ibarra, John A. Koski, Russell F. Warren, TISSUE ENGINEERING MENISCUS Orthopedic Clinics of North America. ,vol. 31, pp. 411- 418 ,(2000) , 10.1016/S0030-5898(05)70160-7
Moreica B. Pabbruwe, Wael Kafienah, John F. Tarlton, Sanjay Mistry, Dennis J. Fox, Anthony P. Hollander, Repair of meniscal cartilage white zone tears using a stem cell/collagen-scaffold implant. Biomaterials. ,vol. 31, pp. 2583- 2591 ,(2010) , 10.1016/J.BIOMATERIALS.2009.12.023
William G. Rodkey, J. Richard Steadman, Shu-Tung Li, A clinical study of collagen meniscus implants to restore the injured meniscus Clinical Orthopaedics and Related Research. ,vol. 367, ,(1999) , 10.1097/00003086-199910001-00027
Steven P. Arnoczky, Building a meniscus. Biologic considerations. Clinical Orthopaedics and Related Research. ,vol. 367, ,(1999) , 10.1097/00003086-199910001-00024
Pia Margarethe Gebhard, Angelika Gehrsitz, Brigitte Bau, Stephan Söder, Wolfgang Eger, Thomas Aigner, Quantification of expression levels of cellular differentiation markers does not support a general shift in the cellular phenotype of osteoarthritic chondrocytes. Journal of Orthopaedic Research. ,vol. 21, pp. 96- 101 ,(2003) , 10.1016/S0736-0266(02)00094-3