A method for the preparation of a high-temperature stable oxygen-carrier-containing pharmaceutical composition and the use thereof

摘要: A high temperature-stable and highly purified cross-linked (optionally ≧70% β-β linked) tetrameric hemoglobin with efficiency of oxygen delivery suitable for use in mammals without causing renal injury vasoconstriction is provided. The dimeric form degenerated purification processes are performed on red blood cells from whole blood. Controlled hypotonic lysis an instant cytolysis apparatus prevents white cells. Nucleic acids phospholipids impurities not detected. Blocking reactive sulfhydryl groups by a reagent oxygenated environment. Flowthrough column chromatography removes different plasma protein impurities. N-acetyl cysteine added to the maintain low level met-hemoglobin. stabilized preserved infusion bag aluminum overwrap prevent formation inactive met-hemoglobin intrusion. product finds tissue oxygenation cancer treatment.