Autoantibody to alanyl-tRNA synthetase in patients with idiopathic pulmonary fibrosis.
作者： Toru TAKAHASHI , Ikuo WADA , Yoshinori OHTSUKA , Mitsuru MUNAKATA , Yukihiko HOMMA
DOI: 10.1111/J.1440-1843.2007.01140.X
关键词: Autoimmunity 、 Immunology 、 Transfection 、 Immunostaining 、 Immunohistochemistry 、 Pathogenesis 、 Molecular biology 、 Autoantibody 、 Medicine 、 Idiopathic pulmonary fibrosis 、 Antigen
摘要: Background and objectives: The pathogenesis of IPF is unknown it hypothesized that immunological responses are involved. purpose this study was to detect autoantibodies in patients identify the relevant antigens. Methods: Sera from 37 healthy subjects 22 who had no clinical symptoms collagen vascular disease were examined for immunostaining A549 human type II cells lung tissue. Immunoprecipitation proteome analysis performed antigen. Results: Fifty per cent patient sera none control exhibited positive staining. 10 showed immunohistochemistry immunoprecipitated a 110-kDa protein cell lysate. only two 41 with immunoreactivity. Proteome using tandem mass spectrometry revealed alanyl-tRNA synthetase. Transfection cDNA enzyme into CHO-K1 conferred staining on these patients' IgG. 135-kDa fusion consisting 108-kDa 27-kDa YFP lysate transfected by In addition, significantly inhibited activity synthetase. Conclusion: A significant number possess circulating against synthetase, suggesting involvement an autoimmune background IPF.
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