Anti-inflammatory Effect of Perilla frutescens (L.) Britton var. frutescens Extract in LPS-stimulated RAW 264.7 Macrophages
作者： Hyun-Ah Lee , Ji-Sook Han
DOI: 10.3746/PNF.2012.17.2.109
关键词: RAW 264.7 Cells 、 Catalase 、 Anti-inflammatory 、 Antioxidant 、 Superoxide dismutase 、 Perilla frutescens 、 Traditional medicine 、 Perilla 、 Glutathione peroxidase 、 Medicine
摘要: This study was designed to investigate the inhibitory effects of Perilla frutescens (L.) Britton var. extract on production inflammation-related mediators (NO, ROS, NF-κB, iNOS and COX-2) pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) in lipopolysaccharide-stimulated RAW 264.7 macrophages. frutescents air-dried extracted with ethanol. The dose-dependently decreased generation intracellular reactive oxygen species increased antioxidant enzyme activities, such as superoxide dismutase, catalase glutathione peroxidase lipopolysaccharide stimulated Also, suppressed NO cells. expressions IL-1β IL-6), COX-2 were inhibited by treatment extract. Thus, this shows could be useful for inhibition inflammatory process.
koreascience.or.kr参考文章(40)
Szabó C, Alterations in nitric oxide production in various forms of circulatory shock. New Horizons-the Science and Practice of Acute Medicine. ,vol. 3, pp. 2- 32 ,(1995)
Hugo Aebi, Catalase in vitro Methods in Enzymology. ,vol. 105, pp. 121- 126 ,(1984) , 10.1016/S0076-6879(84)05016-3
Chung Oui Hong, Sang Yul Park, Hyun Sun Lee, Hyun Ah Lee, Sung Yong Yang, Kwang Won Lee, Sung Yu Hong, Ho Jung Lee, Quantification of Caffeic Acid and Rosmarinic Acid and Antioxidant Activities of Hot-water Extracts from Leaves of Perilla frutescens Korean Journal of Food Science and Technology. ,vol. 41, pp. 302- 306 ,(2009)
Barry Halliwell, J. Robin Hoult, David R. Blake, Oxidants, inflammation, and anti-inflammatory drugs. The FASEB Journal. ,vol. 2, pp. 2867- 2873 ,(1988) , 10.1096/FASEBJ.2.13.2844616
Akira Murakami, Chemoprevention with phytochemicals targeting inducible nitric oxide synthase. Food factors for health promotion. ,vol. 61, pp. 193- 203 ,(2009) , 10.1159/000212751
S.L. Weinstein, J.S. Sanghera, K Lemke, A.L. DeFranco, S.L. Pelech, Bacterial lipopolysaccharide induces tyrosine phosphorylation and activation of mitogen-activated protein kinases in macrophages. Journal of Biological Chemistry. ,vol. 267, pp. 14955- 14962 ,(1992) , 10.1016/S0021-9258(18)42133-3
Linghua Meng, Yves Lozano, Emile Gaydou, Bin Li, Antioxidant activities of polyphenols extracted from Perilla frutescens varieties. Molecules. ,vol. 14, pp. 133- 140 ,(2008) , 10.3390/MOLECULES14010133
Andrew Paul, Ana Cuenda, Clare E. Bryant, Jo Murray, Edwin R. Chilvers, Philip Cohen, Gwyn W. Gould, Robin Plevin, Involvement of Mitogen-Activated Protein Kinase Homologues in the Regulation of Lipopolysaccharide-Mediated Induction of Cyclo-oxygenase-2 but not Nitric Oxide Synthase in RAW 264.7 Macrophages Cellular Signalling. ,vol. 11, pp. 491- 497 ,(1999) , 10.1016/S0898-6568(99)00018-2
Richard A. Lawrence, Raymond F. Burk, Glutathione peroxidase activity in selenium-deficient rat liver Biochemical and Biophysical Research Communications. ,vol. 71, pp. 952- 958 ,(1976) , 10.1016/0006-291X(76)90747-6
Wojciech Król, Zenon P. Czuba, Michael D. Threadgill, Bernadette D.M. Cunningham, Grazyna Pietsz, Inhibition of nitric oxide (NO•) production in murine macrophages by flavones Biochemical Pharmacology. ,vol. 50, pp. 1031- 1035 ,(1995) , 10.1016/0006-2952(95)00237-T