TEAD4, YAP1 and WWTR1 prevent the premature onset of pluripotency prior to the 16-cell stage.

摘要: In mice, pluripotent cells are thought to derive from buried inside the embryo around 16-cell stage. Sox2 is only pluripotency gene known be expressed specifically within at this To understand how established, we therefore investigated mechanisms regulating initial activation of expression. Surprisingly, expression initiated normally in absence both Nanog and Oct4 (Pou5f1), highlighting differences between stem cell models pluripotency. However, observed precocious ectopic prior stage Yap1, Wwtr1 Tead4 Interestingly, repression premature was sensitive LATS kinase activity, even though proteins do not limit activity TEAD4, YAP1 WWTR1 during these early stages. Finally, present evidence for direct transcriptional by YAP1, TEAD4. Taken together, our observations reveal that, while embryos initially competent express as four-cell stage, prevents Sox2, thereby restricting program when first created.