Gamma-irradiation of deoxyribonucleic acid in dilute solutions. I. A sensitive method for detection of single-strand breaks in polydisperse DNA samples.
作者： William C. Summers , Waclaw Szybalski
DOI: 10.1016/0022-2836(67)90264-1
关键词: Cysteine 、 Radiosensitivity 、 Ionizing radiation 、 Thymine 、 Bromouracil 、 Biochemistry 、 Cysteamine 、 Biophysics 、 Chemistry 、 DNA 、 Molecule
摘要: Abstract A relatively simple and rapid method has been developed which allows quantitation of the rate production single-strand scissions in polydisperse populations DNA molecules. This method, is based on determination progressive loss renaturability (zippering-up) cross-linked DNA, was applied as a quantitative assay strand breakage by ionizing radiation DNase I. Radiosensitivity to proportional molecular size but independent average base composition or substitution bromouracil for thymine. The protective effects cysteine cysteamine, self-protection at higher concentrations were demonstrated.
osti.gov
sci-hub.se 参考文章(34)
W. R. Guild, The radiation sensitivity of deoxyribonucleic acid. Radiation Research. ,vol. 3, pp. 257- 269 ,(1963) , 10.2307/3583688
PD LAWLEY, P BROOKES, Further studies on the alkylation of nucleic acids and their constituent nucleotides Biochemical Journal. ,vol. 89, pp. 127- 138 ,(1963) , 10.1042/BJ0890127
Per-Åke Albertsson, Partition studies on nucleic acids Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. ,vol. 103, pp. 1- 12 ,(1965) , 10.1016/0005-2787(65)90537-X
Waclaw Szybalski, Zofia Opara-Kubinska, Zofia Kurylo-Borowska, Genetic transformation studies: III. Effect of ultraviolet light on the molecular properties of normal and halogenated deoxyribonucleic acid Biochimica et Biophysica Acta. ,vol. 72, pp. 298- 309 ,(1963) , 10.1016/0006-3002(63)90245-2
Walter F. Bodmer, A. T. Ganesan, BIOCHEMICAL AND GENETIC STUDIES OF INTEGRATION AND RECOMBINATION IN BACILLUS SUBTILIS TRANSFORMATION. Genetics. ,vol. 50, pp. 717- 738 ,(1964) , 10.1093/GENETICS/50.4.717
O. Vos, RADIATION PROTECTION BY A NUMBER OF SUBSTANCES PREVENTING FREEZING DAMAGE. I. PROTECTION OF MAMMALIAN CELLS IN VITRO. International Journal of Radiation Biology. ,vol. 9, pp. 133- 142 ,(1965) , 10.1080/09553006514550151
Norman Strauss, Configuration of Transforming Deoxyribonucleic Acid During Entry into Bacillus subtilis Journal of Bacteriology. ,vol. 89, pp. 288- 293 ,(1965) , 10.1128/JB.89.2.288-293.1965
Bernard E. Reilly, John Spizizen, Bacteriophage Deoxyribonucleate Infection of Competent Bacillus subtilis1 Journal of Bacteriology. ,vol. 89, pp. 782- 790 ,(1965) , 10.1128/JB.89.3.782-790.1965
P Donini, H.T Epstein, SENSITIVITY TO X RAYS AND ULTRAVIOLET LIGHT OF INTRACELLULAR PHAGE $alpha$ Virology. ,vol. 26, pp. 359- 367 ,(1965) , 10.1016/0042-6822(65)90283-7
Agnar P. Nygaard, B.D. Hall, A method for the detection of RNA-DNA complexes Biochemical and Biophysical Research Communications. ,vol. 12, pp. 98- 104 ,(1963) , 10.1016/0006-291X(63)90242-0