Irradiation induces regionally specific alterations in pro-inflammatory environments in rat brain.
作者： Won Hee Lee , William E. Sonntag , Matthew Mitschelen , Han Yan , Yong Woo Lee
DOI: 10.3109/09553000903419346
关键词: NFKB1 、 Downregulation and upregulation 、 Tumor necrosis factor alpha 、 Transcription factor 、 Internal medicine 、 Molecular biology 、 NF-κB 、 CREB 、 Endocrinology 、 Messenger RNA 、 Inflammation 、 Biology
摘要: Purpose: Pro-inflammatory environments in the brain have been implicated onset and progression of neurological disorders. In present study, we investigate hypothesis that irradiation induces regionally specific alterations cytokine gene protein expression.Materials methods: Four month old F344 × BN rats received either whole with a single dose 10 Gy γ-rays or sham-irradiation, were maintained for 4, 8, 24 h following irradiation. The mRNA expression levels pro-inflammatory mediators analysed by real-time reverse transcriptase-polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA), immunofluorescence staining. To elucidate molecular mechanisms irradiation-induced inflammation, effects on DNA-binding activity transcription factors also examined.Results: A significant marked up-regulation med...
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