8-Cl-cAMP and tiazofurin affect vascular endothelial growth factor production and glial fibrillary acidic protein expression in human glioblastoma cells.

摘要: Compounds that could block tumor angiogenesis and induce cell differentiation in malignant gliomas represent a very valuable tool anticancer treatments. In this paper, we demonstrate more selective drugs, which interfere with specific cellular targets, treat glioma effectively. 8-Cl-cAMP tiazofurin (TR) are site-specific analogs selectively inhibit PKAI IMP dehydrogenase, directly involved proliferation apoptosis, mediate the mitogenic effects of different oncogenes growth factors. study, have examined influence TR on production an angiogenic factor [vascular endothelial (VEGF)] by human glioblastoma U251 MG cells, as well their expression differentiating marker [glial fibrillary acidic protein (GFAP)]. Using assay, VEGF enzyme-linked immunoassay GFAP immunocytochemistry demonstrated these compounds. Our results decrease under both agents cells increase change morphology, becoming differentiated. These findings also suggest may potential for further investigation antiangiogenic differentiational role disease such gliomas.