Comparison of BALB/c and B6-albino mouse strain blastocysts as hosts for the injection of C57BL6/N-derived C2 embryonic stem cells
作者： Tuija M. Alcantar , Rhonda Wiler , Xin Y. Rairdan
DOI: 10.1007/S11248-016-9937-5
关键词: Genetically modified mouse 、 BALB/c 、 Andrology 、 Cell culture 、 Chimera (genetics) 、 Virology 、 Blastocyst 、 Embryonic stem cell 、 Cell 、 Germline 、 Biology
摘要: Embryonic stem (ES) cells from a C57BL/6N (B6N) background injected into B6(Cg)-Tyrc-2J/J (B6-albino) recipient blastocysts are commonly used for generating genetically modified mouse models. To understand the influence of blastocyst strain on germline transmission, BALB/cAnNTac and B6-albino transmission rates were compared using C57BL6/N-derived C2 ES cell line. A total 92 clones 27 constructs injected. We yield, birth rate, chimera formation high-percentage (>50 %) male rate. For we analyzed 24 19 constructs, which generated chimeras both donor strains. hosts resulted in higher mean yields per than did BALB/c ones (3.6 vs. 2.5). However, rate (36 %), (50 42 (10 8 (65 49 respectively. Our data suggest that is suitable host has an advantage over receiving injection cells.
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