Potential action of copper surfaces on meticillin-resistant Staphylococcus aureus.
作者： L. Weaver , J.O. Noyce , H.T. Michels , C.W. Keevil
DOI: 10.1111/J.1365-2672.2010.04852.X
关键词: Cell wall 、 Copper 、 Propidium iodide 、 Staphylococcus aureus 、 Agarose gel electrophoresis 、 Cell membrane 、 Cellular respiration 、 DNA damage 、 Microbiology 、 Biology
摘要: Aims: Studies to date have shown rapid killing of bacterial cells when exposed copper surfaces. The mechanistic action on is so far unknown. Methods and Results: To investigate potential mechanisms involved, meticillin-resistant Staphylococcus aureus (MRSA) (107 CFU) were inoculated onto coupons or stainless steel stained with either the viability fluorophore 5-cyano-2,3-ditolyl tetrazolium (CTC), detect respiration, BacLight™ (SYTO9/propidium iodide), determine cell wall integrity. Coupons then observed in-situ using epifluorescence microscopy. In addition, DNA from surfaces was isolated analysed by agarose gel electrophoresis. An effect cellular respiration CTC reduction evident but no membrane integrity (BacLight™) observed. Results isolation indicated a copper-induced detrimental MRSA genomic material as bands after exposure surface. Conclusions: results indicate that rapidly kills compromising damaging DNA, little integrity. Significance Impact study: This research provides explanation in support previous suggestions although do not affect cells, there still antimicrobial effect.
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