An atomic force microscopy investigation of bioadhesive polymer adsorption onto human buccal cells.

摘要: Atomic force microscopy (AFM) was used to examine the buccal cell surface in order image presence of adsorbed bioadhesive polymers identified from previous work. Isotonic saline solution (5 ml) containing either polycarbophil (pH 7.6), chitosan 4.5) or hydroxypropyl methylcellulose 7.6) (0.5% w/v) exposed freshly collected cells (ca. 48×104 cells/test) for 15 min at 30°C. The were then rinsed with a small volume double distilled water, allowed air-dry on freshy cleaved mica and imaged using contact mode AFM. Untreated showed relatively smooth characteristics, many ‘crater-like’ pits indentations spread over surfaces. Cells that had been treated all investigated appeared have lost crater indentation characteristic gained higher roughness. These results suggest polymer chains onto Quantitative analysis topography significant increases (P<0.05) arithmetic roughness average (Ra) surfaces respect untreated control specimens. changes indicate polymer, confirming This study demonstrates suitability AFM as powerful sensitive technique detecting imaging present mucosal