Differential activation of pp60(c-src) and pp62(c-yes) in human colorectal carcinoma liver metastases.

摘要: pp60(c-src) and pp62(c-yes) are protein tyrosine kinases whose specific activities increased in primary colorectal carcinomas. Activity of is further liver metastases. This study was undertaken to compare expression activity human carcinoma metastases determine the potential prognostic significance differences activation these two kinases. The kinase levels relative those normal colonic mucosa were determined using an immune complex assay immunoblot analysis tissue specimens from 22 patients with synchronous metastatic disease 9 metachronous Of colon tumors, 64% tumors contained elevated both pp62(c-yes). For metastases, however, only 10% had kinases, 61% only, 23% only. Analysis demonstrated that 71% patients, either or decreases tumor. Protein carcinomas remained unchanged mucosa. These results demonstrate differential regulation occurs during tumor progression. Patients have biologically more aggressive a worse prognosis than without their (median survival, 13 months versus 30 months, P < 0.005, Wilcoxon signed rank test). also for (P 0.05,