Insulin-like growth factor I is the key growth factor in serum that protects neuroblastoma cells from hyperosmotic-induced apoptosis.

摘要: Neuroblastoma is a childhood tumor of the peripheral nervous system that remains largely uncurable by conventional methods. Mannitol induces apoptosis in neuroblastoma cell types and insulin-like growth factor I (IGF-I) protects these cells from hyperosmotic-induced affecting apoptosis-regulatory proteins. In current study, we investigate factors enable SH-SY5Y to survive presence an apoptotic stimulus. When are exposed high mannitol concentrations, more than 60% within 48 h. Normal CS prevents dose-dependent manner, with 0.6% protecting 50% cells, 3% rescuing 70% apoptosis. Serum also delays commitment point for fro m9ht o 35 Asurvey several factors, including epidermal (EGF), platelet-derived (PDGF), nerve (NGF), fibroblast (FGF), IGF-I reveals component serum necessary protection death. Mitochondrial membrane depolarization occurs greater 40% after exposure caspase-3 activation increased conditions 9 blocks both mitochondrial normally induced hyperosmotic treatment cells. Our results suggest (1) key death (2) acts upstream mitochondria caspases prevent human neuroblastoma. J. Cell. Physiol. 182:24 ‐32, 2000. © 2000 Wiley-Liss, Inc.